Cdna library preparation

Details Cdna library preparation Cdna library preparation

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2620081

New biochemical protocols for high throughput processing of mRNA samples into cDNA libraries with adaptor sequences compatible with automated sequencing systems are provided. The provided methods produces cDNA libraries which do not have 3' bias 5 associated with current cDNA library production methods. New methods for the production of DNA libraries from DNA are also provided.

L'invention concerne de nouveaux protocoles biochimiques de transformation à haut rendement d'échantillons d'ARNm en bibliothèques d'ADNc avec des séquences adaptatrices compatibles avec des systèmes de séquençage automatisés. Les méthodes de l'invention produisent des bibliothèques d'ADNc dont la distorsion de 3' à 5' n'est pas associée à des méthodes courantes de production de bibliothèques d'ADNc. On décrit de nouvelles méthodes de production de bibliothèques d'ADN à partir de l'ADN.

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