Cdr-grafted type iii anti-cea humanized mouse monoclonal...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/13 (2006.01) A61K 47/48 (2006.01) A61K 49/00 (2006.01) A61K 51/10 (2006.01) C07K 16/30 (2006.01) C07K 16/46 (2006.01) G01N 33/574 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2200868

A humanized monoclonal antibody, comprising the complementarity-determining regions of a parental murine Class III, anti-CEA monoclonal antibody engrafted to the framework regions of a heterologous antibody, wherein the humanized monoclonal antibody retains the binding specificity of, but is less immunogenic in a heterologous host than, the parental murine monoclonal antibody. A preferred murine Class III, anti-CEA monoclonal antibody is the MN-14 antibody and the preferred heterologous antibody is from a human. Also provided are DNA constructs and vectors for producing the humanized monoclonal antibodies, and diagnostic and therapeutic conjugates using same.

Anticorps monoclonal humanisé comprenant les régions déterminantes de la complémentarité d'un anticorps monoclonal murin anti-ACE de classe III greffé aux régions de charpente d'un anticorps hétérologue, où l'anticorps monoclonal humanisé conserve la spécificité de liaison de l'anticorps monoclonal murin, mais est moins immunogène dans un hôte hétérologue. Un anticorps monoclonal murin anti-ACE de classe III de prédilection est le MN-14 et l'anticorps hétérologue de prédilection provient d'un être humain. Sont également fournis des produits de synthèse de l'ADN et des vecteurs pour produire les anticorps monoclonaux humanisés, de même que des conjugués diagnostiques et thérapeutiques y faisant appel.

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