Cell analysis and sorting apparatus for manipulation of cells

G - Physics – 01 – N

Patent

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G01N 15/14 (2006.01) G01N 30/00 (2006.01)

Patent

CA 2390375

A cell analysis and sorting apparatus is capable of monitoring over time the behaviour of each cell in a large population of cells. The cell analysis and sorting apparatus contains individually addressable cell locations. Each location is capable of capturing and holding a single cell, and selectively releasing that cell from that particular location. In one aspect of the invention, the cells are captured and held in wells, and released using vapor bubbles as a means of cell actuation. In another aspect of the invention, the cells are captured, held and released using electric fields traps.

L'invention concerne un appareil d'analyse et de tri de cellules pouvant surveiller, au fil du temps, le comportement de chaque cellule au sein d'une grande population de cellules. L'appareil d'analyse et de tri de cellules contient des emplacements de cellules portant une adresse distincte. Chaque emplacement peut piéger et retenir une seule cellule, et libérer sélectivement ladite cellule dudit emplacement particulier. Dans un aspect de l'invention, les cellules sont piégées et retenues dans des cupules; elles sont libérées par des bulles de vapeur utilisées comme moyens d'activation. Dans un autre aspect de l'invention, les cellules sont piégées, retenues et libérées au moyen de pièges à champs électriques.

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