C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 3/06 (2006.01) C12M 1/00 (2006.01)
Patent
CA 2397600
The invention concerns a device for cell and tissue culture comprising a closed pressurising circuit (2) designed to deliver a selected gas under selected pressure, at least a culture chamber (6) housing support means (13) for at least a deformable membrane (14) forming in the chamber (6) an interface between first (17) and second parts with variable matching volumes, the first part (17) being designed to receive cells and/or tissues to be cultured and to be supplied with culture fluid by a first reservoir (8), and said second part (16) being designed to be supplied with gas by the pressurising circuit (2) and evacuate at least part of said gas. It further comprises control means (3, 4) capable of determining, on the basis of selected criteria relative to the type of culture to be carried out, the gas to be introduced into the second part (16), its pressure and the duration of said gas introduction, and of controlling access to the first and second parts of the chamber (6) so as to simultaneously control the shape of the membrane (14), the fluid culture supply and the fluid circulation in the first part.
Un dispositif de culture de cellules et tissus comprend un circuit fermé de pressurisation (2) propre à délivrer un gaz choisi sous une pression choisie, au moins une chambre de culture (6) logeant des moyens de support (13) d'au moins une membrane déformable (14) formant dans la chambre (6) une interface entre des première (17) et seconde (16) parties à volumes complémentaires variables, la première partie (17) étant agencée pour recevoir des cellules et/ou des tissus à cultiver et pour être alimentée en fluide de culture par un premier réservoir (8), et ladite seconde partie (16) étant agencée pour être alimentée en gaz par le circuit de pressurisation (2) et évacuer une partie au moins de ce gaz. Il comprend en outre des moyens de commande (3, 4) capables de déterminer, en fonction de critères choisis relatifs au type de culture à effectuer, le gaz à introduire dans la seconde partie (16), sa pression et la durée d'introduction de ce gaz, et de contrôler les accès aux première et seconde parties de la chambre (6) de manière à gérer conjointement la forme de la membrane (14), l'alimentation en fluide de culture et la circulation de ce fluide dans la première partie.
Sarem Farzin
Sarem Damerdji Leila-Ouassila
Lesperance & Martineau S.e.n.c.
Sarem Farzin
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