Cell and tissue culture device with enhanced culture fluid flow

Details Cell and tissue culture device with enhanced culture fluid flow Cell and tissue culture device with enhanced culture fluid flow

C - Chemistry, Metallurgy

12

M

C12M 1/00 (2006.01) B65D 88/62 (2006.01)

Patent

CA 2407012

The invention concerns a device for cell and tissue culture comprising several culture wells (18-I) housing the cells or tissues to be cultured, first (2) and second (25) reservoirs and linking means (20, 23, 24) coupled to the wells and the reservoirs enabling a culture fluid to circulate from one reservoir to the other via the wells. Each reservoir (20, 25) houses at least a soft pouch (6, 7, 27, 29) whereof one at least is capable of receiving the culture fluid. The device further comprises at least a control module (50, 150) supplying first and/or second sequences of external pressures to be applied, respectively, on the pouches of the first and second reservoirs, and pressurising means (41, 46-49) designed to apply to the pouches pressures defined by said first and second sequences supplied by the control module.

Un dispositif de culture de cellules et tissus comprend plusieurs puits de culture (18-I) logeant des cellules ou des tissus à cultiver, de premier (2) et second (25) réservoirs, et des moyens de liaison (20, 23, 24) couplés aux puits et aux réservoirs pour permettre la circulation d'un fluide de culture d'un réservoir à l'autre via les puits. Chaque réservoir (20, 25) loge au moins une poche souple (6, 7; 27, 29), dont l'une au moins est propre à recevoir le fluide de culture. Le dispositif comprend en outre au moins un module de commande (50, 150) fournissant de première et/ou de seconde séquences de pressions externes à appliquer, respectivement, sur les poches des premier et seconds réservoirs, et des moyens de pressurisation (41, 46-49) agencés pour appliquer aux poches les pressions définies par lesdites première et seconde séquences fournies par le module de commande.

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