Cell and transgenic animal modelling human antigenic...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) A01K 67/027 (2006.01) A61K 35/14 (2006.01) A61K 48/00 (2006.01) C07K 14/705 (2006.01) C07K 14/74 (2006.01) C12N 15/85 (2006.01) G01N 33/50 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2453928

The invention concerns an isolated animal cell comprising at least a transgene including at least a nucleotide sequence coding for at least a human polypeptide involved in the recognition and/or antigenic activation by T cells. The invention is characterised in that said cell, or a progeny of said cell, expresses at least all or part of the or said human polypeptide(s), and the homologous endogenous animal gene coding for an animal polypeptide homologous with said human peptide is invalid. The invention also concerns the corresponding transgenic animal. The cell and the transgenic animal of the invention can be used in a method for screening compounds which modulate an immune response in humans. The invention further concerns the use of the inventive cell as cell rendered autologous or tolerated by the immune system.

L'invention se rapporte à une cellule animale, isolée, comprenant au moins un transgène comprenant au moins une séquence nucléotidique codant pour au moins un polypeptide humain impliqué dans la reconnaissance et/ou dans l'activation antigénique par les cellules T, caractérisée en ce que ladite cellule, ou une descendante de ladite cellule, exprime au moins tout ou partie du ou desdits polypeptide(s) humain(s) , et caractérisée en ce que le gène animal endogène homologue codant pour un polypeptide animal homologue audit peptide humain est invalide. L'invention porte également sur l'animal transgénique correspondant. La cellule et l'animal transgénique selon l'invention peuvent être mis en oeuvre dans un procédé de criblage de composés qui modulent une réaction immune chez l'homme. L'invention porte également sur l'utilisation de la cellule selon l'invention comme cellule rendue autologue ou tolérée par le système immunitaire.

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