Cell chemotactic factor (cctf) originating in mammalian...

C - Chemistry – Metallurgy – 07 – K

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C07K 14/52 (2006.01) A61K 35/32 (2006.01) A61K 38/18 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2300925

A cell chemotactic factor (CCTF) originating in mammalian tooth precement or cement which has a molecular weight of 67,000 ~ 1,000 determined by SDS-PAGE. A method which comprises taking up the precement or cement from extracted mammalian teeth, immersing the same in physiological saline optionally containing collagenase, and purifying the above CCTF from the resultant eluate by molecular weight fractionation, ion exchange adsorption chromatography and hydroxy-apatite adsorption chromatography.

La présente invention concerne un facteur chimiotactique cellulaire ou "CCTF" (cell chemotactic factor) issu du cément ou précément dentaire des mammifères et qui présente une masse moléculaire de 67.000 ± 1.000 mesuré selon le procédé SDS-PAGE. L'invention concerne également un procédé consistant à prélever de ce cément ou précément de dents extraites de mammifères, à l'immerger dans un sérum physiologique contenant éventuellement une collagénase, et à purifier le facteur CCTF considéré de l'éluat résultant par fractionnement par masses moléculaires, chromatographie par adsorption sur résine d'échange d'ions, et chromatographie sur hydroxyapatite.

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