Cell co-culture for hair follicle production

Details Cell co-culture for hair follicle production Cell co-culture for hair follicle production

C - Chemistry, Metallurgy

12

N

C12N 5/06 (2006.01)

Patent

CA 2646352

This invention relates to methods of producing hair folliclesin vitro, compositions for producing hair follicles in vitro, in vitro produced hair follicles, methods of providing an in vitro produced hair shaft at an interfollicular or intrafollicular site, methods of treating hair loss by providing an in vitro produced hair shaft at an interfollicular or intrafollicular site and assays for studying the effect of test agents on hair biology. The invention also provides the similar methods and products which are, or use, immature follicles ("defined herein as proto- hairs").The invention provides a method for in vitro production of a hair follicle or a proto-hair comprising co-culturing dermal papilla cells with keratinocytes, and optionally with melanocytes.

Cette invention concerne des procédés de production in vitro de follicules pileux, des compositions servant à produirein vitro des follicules pileux, des follicules pileux produits in vitro, des procédés consistant à placer une tige de cheveu produite in vitro au niveau d'un site interfolliculaire ou intrafolliculaire, des procédés de traitement de la chute des cheveux en plaçant une tige de cheveu produite in vitro au niveau d'un site interfolliculaire ou intrafolliculaire et des essais servant à étudier l'effet d'agents testés sur la biologie du cheveu. L'invention concerne également des procédés similaires et des produits similaires lesquels sont ou utilisent des follicules immatures (définis ici en tant que "proto-cheveux"). L'invention concerne un procédé pour la production in vitro d'un follicule pileux ou d'un proto-cheveu consistant à effectuer la co-culture de cellules de bulbe pileux avec des kératinocytes et éventuellement des mélanocytes.

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