C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 1/02 (2006.01) C12N 5/00 (2006.01)
Patent
CA 2372485
Methods are disclosed for using paramagnetic particles to concentrate or harvest cells. Methods are also disclosed for clearing a solution of disrupted biological material, such as a lysate of cells or a homogenate of mammalian tissue. Methods are also disclosed for using paramagnetic particles to isolate target nucleic acids, such as RNA or DNA, from a solution cleared of disrupted biological material using the same type or a different type of paramagnetic particle. Kits are also disclosed for use with the various methods of the present invention. Nucleic acids isolated according to the present methods and using the present kits are suitable for immediate use in downstream processing, without further purification.
L'invention concerne des procédés d'utilisation de particules paramagnétiques pour concentrer ou récolter des cellules. Elle porte également sur des procédés d'élimination du matériau biologique altéré d'une solution, tel qu'un lysat de cellules ou un homogénat de tissu mammalien ainsi que sur des procédés d'utilisation de particules paramagnétiques pour isoler des acides nucléiques cibles, tels que de l'ARN ou de l'ADN, d'une solution débarrassée de matériau biologique altéré, au moyen d'un type de particules paramagnétiques identique ou différent. Des kits à utiliser dans les divers procédés de l'invention sont décrits. Les acides nucléiques isolés selon les procédés de l'invention et au moyen desdits kits peuvent être utilisés immédiatement dans un traitement d'aval, sans purification supplémentaire.
Bitner Rex M.
Butler Braeden L.
Sankbeil Jacqueline
Smith Craig E.
White Douglas H.
Promega Corporation
Shapiro Cohen
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