Cell culture

Details Cell culture Cell culture

C - Chemistry, Metallurgy

12

N

195/145

C12N 5/00 (2006.01) C12M 3/06 (2006.01)

Patent

CA 1041931

ABSTRACT OF THE DISCLOSURE A replaceable, sterilizable, cell growth assembly comprising a chamber structure of gas permeable, liquid im- permeable material having an inner surface to which cells are attachable. The chamber is of tubing configuration and is disposed in a plurality of layers in stacked relation. Spacer structure between the layers of tubing define a a plurality of flow passages between the layers and enable a gaseous environment to bathe the majority of the external surface area of the tubing layers. The chamber also includes an inlet conduit for introducing culture media for flow through the plural layers of the chamber structure and an outlet con- duit for receiving culture media from the chamber structure, and coupling structure for detachably connecting the inlet conduit to a source of culture media and the outlet conduit to an appropriate culture media receptacle.

229974

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