Cell culture

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/00 (2006.01) C12N 5/07 (2010.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) C40B 30/06 (2006.01)

Patent

CA 2501280

A method for determining the effect of a plurality of culture conditions on a cell, comprising the steps of: a) providing a first set of groups of cell units each comprising one or more cells, and exposing said groups to desired culture conditions; (b) pooling two or more of said groups to form at least one second pool; (c) subdividing the second pool to create a further set of groups of cell units; (d) exposing said further groups to desired culture conditions; (e) optionally, repeating steps (b) - (d) iteratively as required; and (f) optionally assessing the effect on a given cell unit of the culture conditions to which it has been exposed.

La présente invention concerne un procédé permettant de déterminer l'effet d'une pluralité de conditions de culture sur une cellule. Ce procédé comporte plusieurs opérations. On commence (a) par prendre un premier ensemble de groupes d'unités cellulaires comprenant chacune au moins une cellule, et on expose ces groupes aux conditions de culture désirées. On regroupe (b) plusieurs de ces groupes pour former au moins un deuxième regroupement. On subdivise (c) le deuxième regroupement pour créer un autre ensemble de groupes d'unités cellulaires. On expose (d) ces autres groupes aux conditions de culture désirées. On repère (e) éventuellement les opérations (b) à (d) de façon itérative selon les besoins. Enfin, on vérifie (f) éventuellement, sur une unité cellulaire, l'effet des conditions de culture auxquelles elle a été exposée.

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