Cell culture medium

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/08 (2006.01) C12N 5/06 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2123094

A basal serum-free medium and a hematopoietic cell growth and differentiation- promoting serum-free medium based thereon are provided for the maintenance, cultivation, growth and differentiation of erythroid progenitor cells, other hematopoietic progenitor cells, and leukemia cells in which the effects of various growth factor compounds can be quantitatively evaluated. Both media are wholly serum-free and contain no intrinsic growth factor compounds. The hematopoietic growth and differentiation medium consists essentially of the basal serum-free medium to which has been added at least one primarily but not exclusively growth promoting agent selected from heme or hemin, interleukin-3 and recombinant human stem cell factor (and optimally all of them), and at least one primarily but not exclusively cell differentiation promoting agent selected from erythropoietin (Epo), insulin-like growth factor (IGF) and a retinoid (and optimally including all of them).

L'invention se rapporte à un milieu sans sérum basique et à un milieu sans sérum promoteur de la différentiation et de la croissance des cellules hématopoïétiques, constitué à partir dudit milieu basique, pour l'entretien, la culture, la croissance et la différentiation de cellules souches d'érythrocytes, d'autres cellules souches hématopoïétiques et de cellules de leucémie dans lesquelles les effets de divers composés de facteurs de croissance peuvent être évalués quantitativement. Les deux milieux décrits ne contiennent pas du tout de sérum ni de composés de facteurs de croissance intrinsèques. Le milieu de différentiation et de croissance hématopoïétique est constitué essentiellement par le milieu basique sans sérum auquel on a ajouté principalement mais pas exclusivement au moins un agent promoteur de croissance choisi parmi l'hème ou l'hémine, l'interleukine-3 et le facteur de cellules souches humaines de recombinaison (et optimalement tous), ainsi que principalement mais pas exclusivement au moins un agent promoteur de différentiation cellulaire choisi parmi l'érythropoïétine (Epo), le facteur de croissance proche de l'insuline (IGF) et une rétinoïde (et optimalement tous).

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