C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/43, 195/46
C12N 1/00 (2006.01) C12N 5/00 (2006.01) C12N 5/06 (2006.01) C12Q 1/04 (2006.01) G01N 33/50 (2006.01)
Patent
CA 2005494
ABSTRACT OF THE DISCLOSURE: The present invention relates to serum-free cell medium developed to support long term multiplication and permanent establishment of a cell line of human liver epithelial cells. The medium may contain an effective cell growth promoting amount of calcium ions; an effec- tive cell growth promoting amount of glucose; an effec- tive amount of insulin to aid cells in glucose uptake; an effective cell growth promoting amount of hydrocortisone; an effective amount of epidermal growth factor to bind epidermal growth factor receptors on cells; an effective amount of transferrin to increase DNA synthesis in cells; an effective amount of cholera toxin to increase DNA synthesis in cells; an effective amount of triiodothyro- nine to increase DNA synthesis in cells; and an effective growth promoting amount of mammalian hormones and mitogenic factors, including lipoprotein, cholesterol, phospholipids and fatty acids.
Cole Katharine H.
Harris Curtis C.
Lechner John F.
Gowling Lafleur Henderson Llp
U.s. Department Of Commerce United States Of America Represented By The Secretary
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