C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 3/06 (2006.01) C12M 1/12 (2006.01) C12M 1/16 (2006.01) C12M 1/20 (2006.01)
Patent
CA 2123586
TITLE CELL CULTURE PLATE WITH A SYSTEM FOR LATERAL DIFFUSION OF MOLECULES THROUGH A BARRIER MEMBRANE ABSTRACT A cell culture plate with a system for lateral diffusion of molecules across a barrier membrane (Figure 1), comprising a base consisting of two or more flat-bottomed open-topped w?lls, denominated the reservoir wells, each containing one or more wells of smaller ??? (dif???? w?lls), likewise open-topped and sharing the same flat base, characterized in that each of the the diffusion w?lls communicates with the larger homologu? which houses it via one or several slits located in the lateral wall(s) of the diffusion well(s), and in that each slit is covered by a semipermeable membrane that permits the passage of soluble substances while retaining insoluble particles and cells; and a removable lid. The cells deposited in the diffusion well(s) may interchange their metabolic products with the corresponding reservoir well containing culture medium or other cells. The problem caused by other devices wherein the diffusion of substances occurs between a higher compartment (which usually contains the cells) and a lower compartment, separated by a barrier membrane, making difficult the microscopic observation of the cells and the manipulation thereof, is thus solved. The field of application include various branches of biology, such as immunology, pha???ology, toxicology, microbiology and parasitology
Robic
Universidade de Santiago de Compostela
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