Cell culture processes

Details Cell culture processes Cell culture processes

C - Chemistry, Metallurgy

12

P

C12P 21/00 (2006.01) C07K 14/00 (2006.01)

Patent

CA 2709890

Culturing heterologous protein-secreting mammalian cells, such as CHO or BHK cells, at 35.1 -36.50C and/or at pH 7.15-7.20 and/or at a dissolved CO2 concentration of 10% or lower. Preferred heterologous proteins are Factor VIII, ADAMTS-13, furin or Factor VII.

L'invention concerne la culture de cellules de mammifères sécrétant des protéines hétérologues, telles que des cellules CHO ou BHK, à une température comprise entre 35,1 et 36,5 °C et/ou à un pH compris entre 7,15 et 7,20 et/ou à une concentration de CO2 dissous inférieure ou égale à 10 %. Les protéines hétérologues préférées sont le facteur VIII, ADAMTS-13, la furine ou le facteur VII.

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