Cell culture systems and methods for organ assist devices

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/071 (2010.01) A61K 35/14 (2006.01) C12M 1/04 (2006.01) C12M 1/12 (2006.01) C12M 3/06 (2006.01)

Patent

CA 2375505

The invention features modular cell culturing devices (1) including one or more flat - plate modules (2), and is based on the discovery that if the flows of liquid medium and oxygenated fluid are separated by a gas-permeable, liquid- impermeable membrane, and the cells are grown attached to the liquid side of the membrane, the device can be used to culture cells with transport of oxygen through the membrane (i.e., direct oxygenation), without regard for the flow rate of the liquid medium passing through the device. The new flow-through cell culturing devices (1) can thus be used to culture cells, e.g., hepatocytes, with high levels of cell function in organ, e.g., liver assist systems, for production of cells, for production of cell-derived products, such as proteins or viruses, or for systems to treat biological liquid to remove toxins, such as ammonia, or add cell-synthesized products, or both.

La présente invention concerne des dispositifs de culture cellulaire modulaires (1) comprenant un ou plusieurs modules de plaques plates (2), reposant sur la découverte que si l'écoulement du milieu liquide et du fluide oxygéné sont séparés par une membrane imperméable aux liquides et perméable aux gaz, et que les cellules sont cultivées attachées du côté liquide de la membrane, ce dispositif peut être utilisé pour cultiver des cellules, le transport d'oxygène se faisant à travers la membrane (c.-à-d., oxygénation directe), sans tenir compte du débit du milieu liquide traversant le dispositif. Ainsi, on peut utiliser les nouveaux dispositifs (1) de culture cellulaire continue pour cultiver des cellules, par exemple, des hépatocytes, avec des niveaux élevés de fonction cellulaire dans l'organe, par exemple des système d'assistance du foie, pour la production de cellules, pour la production de produits provenant de cellules, comme par exemple les protéines ou les virus, ou pour des systèmes destinés à traiter le liquide biologique pour éliminer les toxines, tels que l'ammoniac, ou ajouter des produits synthétisés par des cellules, ou les deux.

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