Cell differentiation based upon particle uptake

Details Cell differentiation based upon particle uptake Cell differentiation based upon particle uptake

C - Chemistry, Metallurgy

12

Q

195/44, 150/8.5

C12Q 1/04 (2006.01) G01N 15/10 (2006.01) G01N 15/14 (2006.01)

Patent

CA 1199598

CELL DIFFERENTIATION BASED UPON PARTICLE UPTAKE Abstract This invention relates to a method of enumerat- ing and isolating mutant cells and other variant cells in a cell population. A cell population is treated with a selective agent which allows non- variant cells to remain phagocytic while rendering non-variant cells non-viable or non-phaqocytic. A suspension of discriminator particles, such as fluorescent or magnetic particles, is mixed and incubated with the cells. The variant cells take up the particles, while the non-variant cells do not take up the particles. The cell population is then analyzed to determine which cells have taken up discriminator particles.

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