Cell extract for cell-free protein synthesis and process for...

Details Cell extract for cell-free protein synthesis and process for... Cell extract for cell-free protein synthesis and process for...

C - Chemistry, Metallurgy

12

P

C12P 21/02 (2006.01) C07K 1/34 (2006.01) C12N 9/00 (2006.01) C12N 15/00 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2483332

It is intended to provide a method of preparing a high-performance cell extract for a cell-free protein synthesis reaction which comprises eliminating a low-molecular weight substance having an activity of inhibiting the protein synthesis from a cell extract having a cell-free protein synthesis activity by dialysis, gel-filtration, ultrafiltration or the like; and a readymade cell extract for cell-free protein synthesis using the same. By effecting the elimination of the low-molecular weight substance inhibiting the protein synthesis in the co-existence of ATP, GTP or an amino acid, the formation of insoluble substances can be prevented and thus the cell extract can be stabilized.

L'invention concerne un procédé de préparation d'un extrait cellulaire haute performance en vue d'effectuer une réaction de synthèse de protéine acellulaire, procédé comprenant l'élimination d'une substance à bas poids moléculaire ayant une activité inhibant la synthèse de protéine à partir d'un extrait cellulaire ayant une activité de synthèse de protéine acellulaire, par dialyse, filtration sur gel, ultrafiltration ou analogue. L'invention concerne en outre un extrait cellulaire tout préparé, en vue d'effectuer la synthèse de protéine acellulaire au moyen de cet extrait. En éliminant la substance à bas poids moléculaire inhibant la synthèse de protéine dans la co-existence de ATP, GTP ou d'un aminoacide, la formation d'une substance insoluble peut être empêchée et, de ce fait, l'extrait cellulaire peut être stabilisé.

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