C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/00 (2006.01) C07K 14/21 (2006.01) C07K 14/27 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2101325
A process for producing a cell-free ice nucleator comprising the steps of: i) culturing a blebing microorganism capable of producing an ice-nucleator protein, the culturing being carried out at a first temperature promoting growth phase of the microor- ganism and being continued until stationary phase: ii) culturing the blebing microorganism, during stationary phase, at a second temperature below the first temperature effective to promote production of active cell-free ice nucleator protein; iii) separating ac- tive cell-free ice-nucleator protein from the microorganism.
Newman Wayne R.
Pochodylo Kristine
Ward Patrick J.
Wight David R.
Genencor International Inc.
Gowling Lafleur Henderson Llp
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