Cell-free methods for detecting protein-ligand interctions

Details Cell-free methods for detecting protein-ligand interctions Cell-free methods for detecting protein-ligand interctions

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12Q 1/00 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2704226

Provided are rapid and sensitive cell-free assay methods for detecting and/or measuring specific bimolecular or higher order interactions via reassembly of a split monomeric reporter protein, and methods of detecting or identifying modulators of such interactions by the effect on the signal provided by the reassembled split reporter protein. This methodology is adaptable to protein-protein, protein-peptide, protein-nucleic acid, protein-methylated or nonmethylated nucleic acid and other small or large molecule ligands and binding proteins.

La présente invention concerne des procédés de dosage acellulaires rapides et sensibles permettant de détecter et/ou de mesurer des interactions bimoléculaires ou d'ordre supérieur via le réassemblage d'une protéine rapporteuse monomérique dédoublée. La présente invention concerne également des procédés de détection ou d'identification de modulateurs de ces interactions par l'effet produit sur le signal fourni par la protéine rapporteuse dédoublée ré-assemblée. Cette méthodologie est adaptable dans le cas protéine-protéine, protéine-peptide, protéine-acide nucléique, protéine-acide nucléique méthylé ou non méthylé et d'autres grands ou petits ligands de molécule et protéines de liaison.

Affiliated with

Also associated with

Rating

Cell-free methods for detecting protein-ligand interctions does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Cell-free methods for detecting protein-ligand interctions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cell-free methods for detecting protein-ligand interctions will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1515331