Cell-free synthesis and isolation of novel genes and...

Details Cell-free synthesis and isolation of novel genes and... Cell-free synthesis and isolation of novel genes and...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 15/67 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2067194

A method for the cell-free synthesis and isolation of novel genes and polypeptides is provided. Within one embodiment, an expression unit is constructed onto which semi-random nucleotide sequences are attached. The semi-random nucleotide se- quences are first transcribed to produce RNA, and then translated under conditions such that polysomes are produced. Poly- somes which bind to a substance of interest are then isolated and disrupted; and the released mRNA is recovered. The mRNA is used to construct cDNA which is expressed to produce novel polypeptides.

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