Cell genomically modified to produce polypeptides with an...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/10 (2006.01) A01H 5/00 (2006.01) A01K 67/027 (2006.01) A61K 39/395 (2006.01) C12N 15/13 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2542130

It is intended to provide a cell capable of regulating the sugar chain structure of a glycoprotein which is a protein product thereof, namely, a cell carrying a knockout genome gene of an enzyme which catalyzes a dehydration reaction of converting GDP-mannose into GDP-4-keto,6-deoxy-GDP mannose; a process for producing a glycoprotein by using the cell; a glycoprotein produced by this process; and its use.

La présente invention concerne une cellule capable de réguler la structure de chaîne de sucre d'une glycoprotéine qui est une protéine de cette structure, à savoir une cellule portant un gène de génome modifié d'une enzyme qui catalyse une réaction de déshydratation de conversion de GDP-mannose en GDP-4-céto, 6-désoxy-GDP mannose. Cette invention concerne aussi un processus de production de glycoprotéine au moyen de cette cellule, une glycoprotéine produite par ce processus et l'utilisation de celle-ci

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