Cell isolation method

Details Cell isolation method Cell isolation method

C - Chemistry, Metallurgy

12

Q

C12Q 1/24 (2006.01) C12Q 1/04 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2397067

The present invention relates to a method of isolating cells from a sample which method comprises binding said cells to a solid support by means of a non- specific ligand immobilised on said solid support, particularly to a method of isolating microorganisms from a sample. Preferred ligands for use in such methods include carbohydrates and derivatives thereof. Also described is a kit for isolating microorganisms from a sample comprising: (a) a solid support having immobilised thereon a ligand which is capable of non-specific binding to microorganisms; (b) means for binding microorganisms to said solid support; optionally (c) means for lysing said cells; and optionally (d) means for binding nucleic acid released from said lysed cells to a solid support.

La présente invention concerne un procédé destiné à isoler des cellules d'un échantillon. Ce procédé comprend la fixation desdites cellules à un support solide au moyen d'un ligand non spécifique immobilisé sur le support solide. L'invention concerne, plus particulièrement, un procédé d'isolement de micro-organismes d'un échantillon. Parmi les ligands préférés utilisés dans de tels procédés, les hydrates de carbone et leurs dérivés. L'invention concerne également une trousse destinée à isoler des micro-organismes d'un échantillon. Cette trousse comprend : (a) un support solide possédant un ligand immobilisé sur sa surface, ce dernier étant capable de se fixer de façon non spécifique à des micro-organismes, (b) des moyens de fixation de micro-organismes audit support, éventuellement (c) des moyens de lyse des cellules, et éventuellement (d) des moyens de fixation des acides nucléiques libérés par les cellules lysées à un support solide.

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