Cell isolation method and uses thereof

Details Cell isolation method and uses thereof Cell isolation method and uses thereof

C - Chemistry, Metallurgy

12

Q

C12Q 1/04 (2006.01) B03C 5/00 (2006.01) C12Q 1/00 (2006.01) G01N 30/00 (2006.01) G01N 33/487 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2440385

This invention relates generally to the field of cell separation or isolation. In particular, the invention provides a method for separating cells, which method comprises: a) selectively staining cells to be separated with a dye so that there is a sufficient difference in a separable property of differentially stained cells; and b) separating said differentially stained cells via said separable property. Preferably, the separable property is dielectrophoretic property of the differentially stained cells and the differentially stained cells are separated or isolated via dielectrophoresis. Methods for separating various types of cells in blood samples are also provided. Centrifuge tubes useful in density gradient centrifugation and dielectrophoresis isolation devices useful for separating or isolating various types of cells are further provided.

L'invention porte de formule générale sur des techniques de séparation ou d'isolement de cellules et en particulier sur un procédé de séparation de cellules comportant les étapes suivantes: (a) coloration sélective des cellules à séparer au moyen d'un colorant de façon à mettre en évidence une différence suffisante les propriétés de séparation des différentes colorées; et (b) séparation des cellules ainsi colorées sur la base desdites propriétés de séparation. Ces propriétés de séparation sont de préférence des propriétés diélectrophorétiques des cellules séparables et isolables par diélectrophorèse. L'invention porte également sur les procédés de séparation de différents types de cellules d'échantillons de sang, et sur des tubes centrifuges pour dispositifs de centrifugation à gradient de densité et d'isolement par diélectrophorèse, permettant de séparer différents types de cellules.

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