Cell line and assay to determine biological activity of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07K 14/705 (2006.01) C12N 5/10 (2006.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2088293

ABSTRACT OF THE INVENTION The present invention relates to an assay and cell lines for determining the biological effects of retinoids. The cell line is derived from a human lung tumour line which is tumorigenic in nude mice and expressed undetectable levels of the .beta. receptor of retinoic acid by introducing in a stable fashion the DNA coding for this receptor. The novel line is non- tumorigenic and grows in culture at a rate which is reduced in the presence of 10-7 M retinoic acid. This line is useful in determining the biological effects of novel retinoids which act in particular by activating RAR.beta..

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