Cell line carrying an excess of mammalian centromeres

Details Cell line carrying an excess of mammalian centromeres Cell line carrying an excess of mammalian centromeres

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2042093

DNA fragments and methods for obtaining them are disclosed which when put into mammalian cells together with a dominant marker gene are able to form functional centromeres. The sequences can be used to generate probes for these centromeres. Cell lines containing the functional centromeres are also provided. Methods are taught for isolating mammalian centromeric DNA as well as for producing cell lines car- rying an excess of mammalian centromeres linked to a dominant selectable marker gene.

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