Cell line, system and method for optical control of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/62 (2006.01) C07K 14/705 (2006.01) C07K 19/00 (2006.01) C12N 15/00 (2006.01) C12N 15/12 (2006.01) C12P 21/00 (2006.01) C12P 21/06 (2006.01) C12Q 1/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2726128

A variety of methods, devices and compositions are implemented for light- activated molecules. One such method is implemented for generating secondary messengers in a cell. A nucleotide sequence for expressing a chimeric light responsive membrane protein (e.g., rhodopsin) is modified with one or more heterologous receptor subunits {e.g., an adrenergic receptor (al-pha1, Beta2)}. The light responsive membrane protein is expressed in a cell for producing a secondary messenger in response to light.

L'invention porte sur une pluralité de procédés, de dispositifs et de compositions qui sont mis en uvre pour des molécules activées par la lumière. Un tel procédé est mis en uvre pour générer des messagers secondaires dans une cellule. Une séquence nucléotidique pour exprimer une protéine de membrane sensible à la lumière chimérique (par exemple la rhodopsine) est modifiée par une ou plusieurs sous-unités de récepteur hétérologues {par exemple un récepteur adrénergique (alpha1, bêta2)}. La protéine de membrane sensible à la lumière est exprimée dans une cellule pour produire un messager secondaire en réponse à la lumière.

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