Cell lineage markers

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/06 (2006.01) C12N 5/10 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2356155

Sox gene expression correlates in general with specific stages during embryogenesis. It has been determined that the expression of Sox genes may be used, as directed herein, to induce or select pluripotent cells which are at least partially committed to a given developmental pathway. There is provided a method for isolating a pluripotent cell which is at least partially committed to a given developmental pathway, comprising the steps of: a) selecting a population of pluripotent cells; b) sorting the cells according to Sox gene expression; and c) isolating those cells which express a given Sox gene.

L'expression du gène Sox est en général corrélée à des étapes spécifiques pendant l'embryogénèse. Il a été déterminé que l'expression des gènes Sox peut être utilisée pour induire ou sélectionner des cellules multipotentes qui sont au moins partiellement attribuées à une voie de développement donnée. L'invention traite également d'un procédé permettant d'isoler une cellule multipotente qui est au moins partiellement attribuée à une voie de développement donnée. Ce procédé consiste à (a) sélectionner une population de cellules multipotentes, (b) trier les cellules en fonction de l'expression du gène Sox; et (c) isoler lesdites cellules qui expriment un gène Sox donné.

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