Cell lines for use in increasing protein yield from a cell...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/85 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2549218

The present invention provides cell lines useful in the production proteins and peptides. The cell lines contain recombinant expression constructs. The recombinant expression construct encodes the STPs consisting of the Cy protein motif and/or an ankyrin-binding protein motif. Each recombinant expression construct also contains an inducible transcription regulation element having for conditional expression of the senescence-triggering factors (STPs).

L'invention concerne des lignées cellulaires utilisées dans la production de protéines et de peptides. Lesdites lignées cellulaires contiennent des constructions d'expression recombinée. Ladite construction code les peptides déclencheurs de sénescence (STP) constitués d'un motif de la protéine Cy et/ou d'un motif de protéine liant l'ankyrine. Chaque construction contient également un élément de régulation de la transcription inductible présentant une expression conditionnelle des facteurs déclencheurs de sénescence (STP).

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