C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/06 (2006.01) C07H 19/00 (2006.01) C07H 21/00 (2006.01) C07K 1/14 (2006.01) C12P 21/06 (2006.01) G01N 33/53 (2006.01)
Patent
CA 2504129
Cell lysis compositions, methods for extracting and isolating proteins and peptides from a host cells using the compositions, kits and apparatus for extracting and isolating protein and peptide molecules from host cells and for detecting for the presence of a protein or peptide. The composition allows for the extraction and isolation of proteins and peptides from host cells without the need for mechanical disruption and with or without isolation of the cells from cell medium. The composition includes at least one surfactant having a hydrophobic-lipophilic balance value in the range from about 11 to about 16; and at least one cell membrane altering compound.
L'invention concerne des compositions de lyse cellulaire, des procédés permettant d'extraire et d'isoler des protéines et des peptides de cellules hôtes au moyen de ces compositions, ainsi que des trousses et un appareil d'extraction et d'isolation de molécules protéiques et peptidiques de cellules hôtes, et de détection de la présence d'une protéine ou d'un peptide. La composition permet d'extraire et d'isoler des protéines et des peptides de cellules hôtes sans recourir à une dislocation mécanique et avec ou sans isolation des cellules du milieu cellulaire. La composition comporte au moins un tensioactif présentant une valeur d'équilibre hydrophobe-lipophile d'environ 11 à environ 16 ; et au moins un composé modifiant la membrane cellulaire.
Bozek Laura L.
Engel Laurie
Johnson Tonny M.
Shultz John W.
Stevens Judith N.
Mbm Intellectual Property Law Llp
Promega Corporation
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