Cell lysis reagent for isolation of rna

Details Cell lysis reagent for isolation of rna Cell lysis reagent for isolation of rna

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 1/06 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2728232

RNA is extracted from cellular material with a reagent that includes heparin, a reducing agent to reduce disulfide bonds, a chelating agent, a buffer, and an alkali metal halide. The reagent does not require the use of organic solvents, and the reagent allows extraction to be performed in a relatively short period of time in comparison to the prior art.

Selon l'invention, de l'ARN est extrait à partir d'un matériel cellulaire par un réactif qui comprend de l'héparine, un agent réducteur pour réduire les liaisons disulfure, un agent chélatant, un tampon et un halogénure de métal alcalin. Le réactif ne requiert pas l'utilisation de solvants organiques, et le réactif permet à l'extraction d'être effectuée dans une période de temps relativement courte en comparaison avec l'état antérieur de la technique.

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