Cell measuring method

G - Physics – 01 – N

Patent

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G01N 15/14 (2006.01) C12Q 1/02 (2006.01) G01N 33/48 (2006.01)

Patent

CA 2581122

A technology capable of measuring cell-related reactions at a time within a wide range of concentration. Cell groups (200) are disposed in a cell-storing space (110) filled with liquid (120). A specific substance is supplied to the liquid in the cell-storing space (110) to form in the liquid (120) the concentration gradient (131) of the specific substance (130) extending from one end (111) of the cell-storing space (110) toward the other end (112). The cell groups in the cell-storing space (110) is picked up by an imaging device (320) and captured into an information processor, and image obtained by the information processor is divided into a plurality of regions along the above concentration gradient, feature amounts are extracted from image portions in respective regions, and information indicating the interrelation between the extracted feature amounts and the concentration gradient is created.

L~invention concerne une technologie capable de mesurer des réactions liées aux cellules à un instant donné sur une large fourchette de concentration. On dispose des groupes de cellules (200) dans un espace de stockage de cellules (110) rempli de liquide (120). On injecte une substance spécifique dans le liquide dans l~espace de stockage de cellules (110) pour constituer dans le liquide (120) le gradient de concentration (131) de la substance spécifique (130) s~étendant depuis une extrémité (111) de l~espace de stockage de cellules (110) vers l~autre extrémité (112). Les groupes de cellules dans l~espace de stockage de cellules (110) sont détectés par un dispositif d~imagerie (320) et capturés dans un processeur d~informations, et l~image obtenue par le processeur d~informations est divisée en une pluralité de régions selon le gradient de concentration ci-dessus, on extrait les quantités caractéristiques des portions d~image dans des régions respectives, et l~on crée des informations indiquant l~interrelation entre les quantités caractéristiques extraites et le gradient de concentration.

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