C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/06 (2006.01)
Patent
CA 2621026
The present invention provides compositions and methods for preparation of a three-dimensional transendothelial cell migration (TEM) assay. These compositions and methods are uniquely suited for the high throughput TEM assay, and for the analysis and identification of TEM mediators which inhibit or stimulate this process. The composition for detecting migration of cells comprises a solid layer comprising collagen gel; a first cellular layer in contact with the solid layer and comprising a first cell type; and a second cell type seeded on top of the first cellular layer. Optionally, gelatin is included in the collagen gel. A 96 well plate format is disclosed, the combination with a high throughput cellular scanner enables high throughput TEM assay.
L'invention concerne des compositions et des méthodes de préparation d'un dosage de migration cellulaire transendothéliale (TEM) tridimensionnelle. Ces compositions et ces méthodes sont spécifiquement adaptées pour un dosage TEM à haut rendement, et pour l'analyse et l'identification de médiateurs TEM inhibant ou stimulant ce processus. La composition permettant de détecter la migration des cellules comprend une couche solide contenant du gel de collagène, une première couche cellulaire en contact avec la couche solide et comprenant un premier type de cellule, et un second type de cellule au-dessus de la première couche cellulaire. De la gélatine est éventuellement incluse dans le gel de collagène. L'invention concerne également un format de plaque de 96 puits, combiné à un dispositif de balayage cellulaire à haut rendement pour obtenir un dosage TEM à haut rendement.
Beletskii Anton
Channavajhala Padma
Yu Liming
Zhao Lihong
Fetherstonhaugh & Co.
Ge Healthcare Bio-Sciences Corp.
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