Cell nuclear transfer

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/87 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2656362

The present invention discloses methods for cell nuclear transfer that comprise for example modification of zona pellucida of an oocyte, and/or sectioning of oocytes into several parts. The present invention also discloses methods for producing a genetically modified non-human mammal. Genetically modified non-human mammals obtainable by the disclosed methods are also within the scope of the present invention. Disclosed are also methods for cryopreservation of cells.

L'invention concerne des méthodes de transfert nucléaire de cellules qui comprend, par exemple, la modification de la zone pellucide d'un oocyte et/ou le découpage d'oocytes en plusieurs parties. L'invention concerne également des méthodes permettant de produire un mammifère non humain génétiquement modifié. L'invention concerne en outre les mammifères non humains génétiquement modifiés obtenus au moyen des méthodes selon l'invention. L'invention concerne enfin des méthodes de cryopréservation de cellules.

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