Cell population with identification codes and method of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/04 (2006.01) C12N 5/10 (2006.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2488233

It is intended to provide a cell population distinguishable from each other based on a difference in luminous signals emitted from luminous bodies wherein the difference in the luminous signals is caused by either or both of (a) 2 or more different luminous characteristics, and (b) 2 or more different luminous sites. The cell population provided with identification codes can be conveniently prepared without resort to any special apparatus and applied to both of a solid phase and a liquid phase in mass screening. Moreover, this cell population is applicable to a screening concerning various properties of cells such as the expression of a target protein.

La présente invention concerne une population de cellules se distinguant les unes des autres sur la base d'une différence dans les signaux lumineux émis par des corps lumineux, la différence entre signaux lumineux venant soit (a) d'au moins deux caractéristiques lumineuses différentes, soit (b) d'au moins deux sites lumineux différents, soit enfin des deux. Cette population de cellules pourvues de codes d'identification, qui s'obtient facilement, sans utilisation d'appareillage spécial, s'applique, pour un criblage de masse, aussi bien à une phase solide qu'à une phase liquide. De plus, cette population de cellules se prête au criblage par rapport à diverses propriétés des cellules telles que l'expression d'une protéine cible.

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