Cell potential measuring electrode and measuring apparatus...

G - Physics – 01 – N

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G01N 27/403 (2006.01) C12M 1/34 (2006.01) G01N 33/487 (2006.01)

Patent

CA 2316213

This invention relates to a low impedance cell potential measuring electrode assembly typically having a number of microelectrodes on an insulating substrate and having a wall enclosing the region including the microelectrodes. The device is capable of measuring electrophysiological activities of a monitored sample using the microelectrodes while cultivating those cells or tissues in the region of the microelectrodes. The invention utilizes independent reference electrodes to lower the impedance of the overall system and to therefore lower the noise often inherent in the measured data. Optimally the microelectrodes are enclosed by a physical wall used for controlling the atmosphere around the monitored sample.

L'invention concerne un ensemble électrode de mesure de potentiel de cellule de faible impédance comprenant généralement un certain nombre de microélectrodes sur un substrat isolant et une paroi enfermant la région contenant les microélectrodes. Le dispositif est capable de mesurer les activités électrophysiologiques d'un échantillon surveillé à l'aide des microélectrodes tout en cultivant ces cellules ou tissus dans la région des microélectrodes. L'invention utilise des électrodes de référence indépendantes pour réduire l'impédance du système global et pour ainsi réduire le bruit souvent inhérent aux données mesurées. De façon optimale, les microélectrodes sont enfermées par une paroi physique utilisée pour commander l'atmosphère autour de l'échantillon surveillé.

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