C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/04 (2006.01) A01N 1/02 (2006.01)
Patent
CA 2399423
A cell-preservation liquid to which neither serum nor a culture medium is added during preservation and yet which shows an excellent preservation performance. Different from dimethyl sulfoxide having been used as an additive in preservation liquids containing serum, the above cell-preservation liquid contains an albumin mixture, which is contained in phosphate buffers employed in preserving germ cells, as the main agent optionally together with auxiliary agents such as phosphate ion, divalent metal ions or monosaccharides. Accordingly, it is unnecessary to add serum or a culture medium to the preservation liquid and thus there arises no risk of denaturing the cells under preservation or being contaminated with unknown viruses. Thus, it becomes possible to provide a preservation liquid, which contains definite components, has highly stable qualities and show an excellent performance of preserving cells, and a method of preserving cells.
L'invention concerne un liquide de conservation de cellules auquel aucun sérum ni milieu de culture n'est ajouté pendant la période de conservation et qui possède cependant d'excellentes propriétés de conservation. A la différence du diméthylsulfoxyde utilisé jusqu'à maintenant comme additif dans les liquides de conservation contenant du sérum, ledit liquide de conservation de cellules contient un mélange d'albumine dans des tampons de phosphate employés dans la conservation de cellules germinales, en tant qu'agent principal éventuellement mélangé à des agents auxiliaires, tels que les ions phosphate, les ions métal divalent ou des monosaccharides. Ainsi, il n'est pas nécessaire d'ajouter de sérum ou de milieu de culture au liquide de conservation et le risque de dénaturation des cellules ou de contamination par des virus inconnus pendant la période de conservation est supprimé. On produit donc un liquide de conservation contenant des composants définis, possédant une stabilité élevée et des propriétés de conservation des cellules excellentes. L'invention porte également sur un procédé de conservation de cellules.
Bamba Kenzo
Kuroiwa Yasuyuki
Sekine Teruaki
Finlayson & Singlehurst
Humantec Ltd.
Lymphotec Inc.
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