Cell preservation method

Details Cell preservation method Cell preservation method

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) C12N 5/0735 (2010.01) A61K 35/12 (2006.01) A61K 35/48 (2006.01) C12N 1/04 (2006.01)

Patent

CA 2569485

The present invention provides a method for freezing a stem cell or a cell derived therefrom, the method including the steps of providing a cell suspension, performing ice nucleation on the cell suspension, and lowering the temperature of the ice nucleated cell suspension to a temperature sufficiently low to allow long term storage of the stem cell. The method is preferably used for the cryopreservation of human embryonic stem cells.

La présente invention concerne une méthode de congélation d'une cellule souche ou d'une cellule dérivée, ladite méthode consistant à préparer une suspension cellulaire, à exécuter une action glaçogène sur la suspension cellulaire, et à baisser la température de la suspension cellulaire soumise à l'action glaçogène jusqu'à atteindre une température suffisamment basse permettant un stockage à long terme de la cellule souche. Ladite méthode est utilisée, de préférence, dans la cryoconservation de cellules souches embryonnaires humaines.

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