Cell separation apparatus and method

B - Operations – Transporting – 01 – D

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B01D 21/26 (2006.01) B01L 3/14 (2006.01) C12M 1/00 (2006.01) C12M 3/00 (2006.01) C12Q 1/24 (2006.01) G01N 33/49 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2198607

Disclosed are methods for enriching specific cell types from cell mixtures. Included in the methods are the use of precisely defined cell separation media, a cell separation apparatus that includes a constriction defining a lower region and a defined cell separation medium. The constriction prevents mixing between the upper and lower portions of the tube. Also disclosed is a density adjusted cell separation technique used to augment the above methods. In addition, the invention includes specific methods for isolating CD34+ hematopoietic progenitor cells, nucleated fetal cells, and breast tumor cells. The isolation methods are useful in various diagnostic and therapeutic regimens.

L'invention concerne des procédés de séparation par enrichissement de types de cellules spécifiques dans des mélanges de cellules. Ces procédés consistent à utiliser des milieux de séparation de cellules définies avec précision, un dispositif de séparation de cellules comportant un rétrécissement définissant une région inférieure, et un milieu de séparation de cellules définies. Le rétrécissement empêche le mélange entre le contenu des parties supérieure et inférieure du tube. L'invention concerne également une technique de séparation de cellules par réglage de densité, qu'on utilise afin d'accroître l'efficacité des procédés précités. De plus, l'invention comprend des procédés spécifiques d'isolation de cellules hématopoïétiques précurseurs CD34?+¿, de cellules foetales nucléées, ainsi que de cellules de tumeur du sein. Ces procédés d'isolation s'utilisent efficacement à des fins diagnostiques et thérapeutiques.

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