Cell separation method

Details Cell separation method Cell separation method

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) C12N 5/078 (2010.01) C12M 3/00 (2006.01) C12N 1/04 (2006.01) C12N 5/00 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2278208

A method for separating cells which involves the steps of introducing a cell- containing liquid containing the cells to be collected together with the cells to be eliminated into cell-capture means capable of substantially capturing the cells to be collected while passing the cells to be eliminated therethrough; discarding the liquid containing the cells to be eliminated from the means; and introducing a liquid having a viscosity of from 5 mPa.s to 500 mPa.s into the cell-capture means to thereby recover the cells to be collected, which have been captured in the cell-capture means, therefrom.

Procédé pour séparer des cellules, qui consiste à introduire, dans un système de capture de cellules, un liquide contenant les cellules destinées à être recueillies et les cellules à éliminer. Le système peut sensiblement capturer les cellules à recueillir tout en laissant passer les cellules à éliminer. Les étapes suivantes consistent à rejeter du système le liquide contenant les cellules à éliminer et à introduire dans ledit système un liquide ayant une viscosité comprise entre 5 et 500 mPa . s, de manière à récupérer les cellules à recueillir qui ont été capturées dans ce système.

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