Cell-specific adenovirus vectors comprising an internal...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/861 (2006.01) A61K 48/00 (2006.01) A61P 35/00 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2404235

Disclosed herein are replication-competent adenovirus vectors comprising co- transcribed first and second genes under transcriptional control of a heterologous, target cell-specific transcriptional regulatory element (TRE), wherein the second gene is under translational control of an internal ribosome entry site. Methods for the preparation and use of such vectors are also provided. The vectors provide target cell-specific virus replication in applications such as cancer therapy and gene therapy.

L'invention concerne des vecteurs adénoviraux pouvant se répliquer, comprenant des premier et second gènes co-transcrits sous le contrôle transcriptionnel d'un élément de régulation transcriptionnelle à spécificité cellulaire cible hétérologue; le second gène étant placé sous le contrôle transcriptionnel d'un site récepteur interne du ribosome. L'invention concerne également des procédés permettant d'élaborer et d'utiliser de tels vecteurs. Ces vecteurs permettent d'obtenir une réplication du virus dans des applications, telles que la thérapie de traitement des cancers et la thérapie génique.

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