Cell usable in serum-free culture and suspension culture and...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/071 (2010.01) A61K 39/12 (2006.01) C07K 14/005 (2006.01) C12N 7/00 (2006.01)

Patent

CA 2401117

A novel MDCK cell-induction line (B-702) which can be cultured without using fetal bovine serum, has been suspended to enable tank culture on the production scale and requires no support in the suspended state; and a process for economically, highly safely and stably producing a virus for a vaccine by using the above cell.

La présente invention concerne une nouvelle lignée d'induction cellulaire MDCK (B-702) pouvant être mise en culture sans utiliser de sérum bovin foetal. La mise en suspension permet une production à grande échelle par culture en bac. Cette culture en suspension ne nécesite aucun substrat. L'invention concerne également un procédé permettant, en utilisant la cellule considérée, de produire de façon économique, très sûre et très stable un virus destiné à un vaccin.

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