Cell wall mutants for delivery of biologically active compounds

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/74 (2006.01) A61K 39/00 (2006.01) A61K 49/00 (2006.01) C12N 9/10 (2006.01) C12N 9/90 (2006.01)

Patent

CA 2492685

The present invention relates to a recombinant microorganism comprising a mutation modulating the expression of a gene involved in the biosynthesis, modification or degradation of a cell wall component or expressing a foreign gene interfering with the biosynthesis, modification or degradation of a cell wall component and comprising an expression vector leading to the production of a polypeptide having a prophylactic or therapeutic activity.The invention further relates to a method for the delivery of polypeptides at mucosal surfaces, comprising administering to a mucosal surface of an individual (human or animal) a composition comprising a recombinant microorganism comprising a mutation modulating the expression of a gene involved in the biosynthesis, modification or degradation of a cell wall component or expressing a foreign gene interfering with the biosynthesis, modification or degradation of a cell wall component and wherein the polypeptide to be delivered is produced by said recombinant microorganism.

La présente invention a trait à un micro-organisme recombinant comportant une mutation modulant l'expression d'un gène impliqué dans la biosynthèse, la modification ou la dégradation d'un constituant de paroi cellulaire ou exprimant un gène étranger perturbant la biosynthèse, la modification ou la dégradation d'un constituant de paroi cellulaire et comportant un vecteur d'expression conduisant à la production d'un polypeptide présentant une activité prophylactique ou thérapeutique. L'invention a également trait à un procédé permettant la délivrance de polypeptides à des muqueuses, comprenant l'administration à une muqueuse d'un sujet (humain ou animal) d'une composition comportant un micro-organisme recombinant comportant une mutation modulant l'expression d'un gène impliqué dans la biosynthèse, la modification ou la dégradation d'un constituant de paroi cellulaire ou exprimant un gène étranger perturbant la biosynthèse, la modification ou la dégradation d'un constituant de paroi cellulaire et dans lequel le polypeptide à délivrer est produit par ledit micro-organisme recombinant.

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