Cells for detection of enteroviruses

Details Cells for detection of enteroviruses Cells for detection of enteroviruses

C - Chemistry, Metallurgy

12

N

C12N 5/10 (2006.01) C12N 7/00 (2006.01) C12Q 1/04 (2006.01) C12Q 1/70 (2006.01) G01N 33/569 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2445358

The invention provides cell lines which are useful for the rapid detection of enteroviruses. In particular, the invention provides transgenic African green monkey kidney cell lines and buffalo green monkey kidney cell lines. The invention provides cell lines which have increased sensitivity to infection by enteroviruses in single-cell type and mixed-cell type cultures compared to other cell types which are currently used for enterovirus detection. The cells of the invention also are permissive to infection by a larger number of enteroviruses as compared to the cell type from which they were derived.

Lignées cellulaires qui sont utiles pour une détection rapide d'entérovirus. En particulier, la présente invention concerne des lignées cellulaires transgéniques de reins de singes verts d'Afrique et des lignées cellulaires transgéniques de reins de singes verts <= buffalo green >=. La présente invention permet d'obtenir deslignées cellulaires qui possèdent une sensibilité accrue aux infections par des entérovirus dans des cultures à un seul type cellulaire et à type cellulaire mélangé, comparées à d'autres types cellulaires qui sont actuellement utilisés pour la détection d'entérovirus. Les cellules selon la présente invention peuvent être infectées par un plus grand nombre d'entérovirus que le type cellulaire dont elles sont dérivées.

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