Cells for drug discovery

Details Cells for drug discovery Cells for drug discovery

C - Chemistry, Metallurgy

12

N

C12N 5/00 (2006.01) C12N 15/00 (2006.01) G01N 33/50 (2006.01) G01N 33/533 (2006.01) G01N 33/554 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2398590

Disclosed herein are compositions and methods useful in screening a compound for its interaction and/or effect with a molecular target and/or cellular process. The invention is based on the construction of fusion proteins between a zinc finger protein designed to recognize a specific promoter and a transcription repressor or activator. Claimed are methods for the screening of substances capable of interacting with a specific cellular target or modulating a cellular pathway, involving cells stably transfected with, and expressing, the fusion protein.

L'invention concerne des compositions et un procédé utiles dans le criblage d'un composé pour déterminer son interaction et/ou son effet par rapport à une cible moléculaire et/ou un processus cellulaire.

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