Cells having a spectral signature, and methods of...

G - Physics – 01 – N

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G01N 33/58 (2006.01) C12N 5/06 (2006.01) G01N 33/50 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2424817

Methods, compositions and articles of manufacture for encoding a cell with semiconductor nanocrystals and/or other fluorophors are provided. The encoded cells can be subjected to functional assays in mixed populations, and an assay result can be determined and associated with individual cells by virtue of their code. The methods are particularly useful in multiplex settings where a plurality of encoded cells are to be assayed. The methods are used in screening methods for G protein coupled receptors (GPCRs), for identifying the ligands and functions of orphan GPCRs, and for screening for modulators of GPCRs. Kits comprising reagents for performing such methods are also provided.

L'invention concerne des procédés, compositions et articles de fabrication destinés à coder une cellule au moyen de nanocristaux semiconducteurs et/ou autres fluorophores. Les cellules codées peuvent être soumises à des analyses fonctionnelles dans des populations mixtes, après quoi un résultat d'analyse peut être déterminé et associé à des cellules individuelles en fonction de leur codage. Lesdits procédés sont particulièrement utiles dans des paramètres multiplexes où une pluralité de cellules codées doit être soumise à une analyse. Ces procédés sont utilisés dans des méthodes de criblage de récepteurs couplés aux protéines-G (RCPGs), pour identifier les ligands et les fonctions des RCPGs orphelins, et pour réaliser le criblage de modulateurs de RCPGs. L'invention concerne également des trousses contenant des réactifs pour mettre en oeuvre de tels procédés.

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