Cellspottm applications

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C12Q 1/02 (2006.01) C40B 30/04 (2006.01) G01N 33/53 (2006.01) G01N 33/564 (2006.01) G01N 33/567 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2657123

A multiplicity of applications of the CellSpotTM assay method are described. Among these applications are extension to integral membrane protein probes, extension to secretion from bacterial cells, identification of antibodies with enhanced affinity, identification of clones with increased secretion levels, and use of massively parallel screening to identify rare efficacious antibodies.

L'invention concerne une multiplicité d'applications du procédé de dosage CellSpotTM. Parmi ces applications, se trouvent l'extension à des sondes de protéine membranaire intégrale, l'extension à la sécrétion à partir de cellules bactériennes, l'identification d'anticorps avec une affinité améliorée, l'identification de clones avec des taux de sécrétion augmentés et l'utilisation d'un criblage parallèle de façon massive pour identifier des anticorps efficaces rares.

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