Cellular and animal models for diseases associated with...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/12 (2006.01) A01K 67/00 (2006.01) C12N 5/06 (2006.01) C12N 5/16 (2006.01) C12N 5/22 (2006.01) C12N 5/26 (2006.01) C12N 15/00 (2006.01) C12N 15/07 (2006.01) C12N 15/87 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2326600

The present invention provides methods for depleting mitochondrial DNA from insulin secreting cells using antiviral compounds, and for producing mitochondrial cytoplasmic hybrid ("cybrid") cells and animals from mitochondrial DNA depleted cells. Also provided are biological models for diseases associated with altered mitochondrial function, including NIDDM, and methods for diagnosis of such diseases and methods for screening agents useful for treating such diseases. Also provided are biological models and methods for evaluating an antiviral compound for its suitability for use in treating a virally-infected patient having a disease associated with impaired insulin secretion, and for evaluating modifications to antiviral compounds in order to determine if such modifications alter (e.g., ameliorate or exacerbate) undesirable side-effects associated with the antiviral compound.

L'invention concerne un procédé pour épuiser l'ADN mitochondrial, contenu dans les cellules sécrétant l'insuline, au moyen de composés antiviraux et pour produire des cellules et des hybrides cytoplasmiques mitochondriaux ("cybrides") à partir de cellules appauvries en ADN mitochondrial. L'invention concerne également des modèles biologiques pour les maladies liées à la modification de la fonction mitochondriale, y compris le diabète sucré non-insulino-dépendant, des procédés pour diagnostiquer ces maladies et des procédés pour cribler les agents utiles pour traiter ces maladies. L'invention concerne en outre des modèles biologiques et des procédés pour évaluer un composé antiviral en matière de son aptitude à être utilisé dans le traitement d'un patient infecté par un virus et souffrant d'une maladie liée à la sécrétion affaiblie d'insuline ainsi que pour évaluer les modifications apportées aux composés antiviraux afin de déterminer si ces modifications changent (en mieux ou en pire) les effets secondaires indésirables liés au composé antiviral.

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