Cellular differentiation process and its use for blood...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/071 (2010.01) C12N 5/0775 (2010.01) C12N 5/0789 (2010.01) C12N 11/00 (2006.01)

Patent

CA 2729074

The present invention relates to the use of specific oxygen concentrations for implementing a process of differenti-ation of stem, provided that said stem cells are not humanembryonic stemcells, and seeded on a support, in an appropriate culture medium, wherein said differentiation leads to: -either a first group of specialized differentiated cells under normoxic conditions, and in an appropriate culture medium, -or a second group of specialized differentiated cells under hypoxic conditions, in a culture medium of the same nature as the one used for obtaining the first group of specialized differentiated cells, said first and second groups of specialized differentiated cells retaining the functional properties of the corresponding specialized differentiated cells respectively obtained through a biological natural process.

La présente invention concerne l'utilisation de concentrations spécifiques en oxygène pour implémenter un procédé de différenciation de cellules souches, à condition que lesdites cellules souches ne soient pas des cellules souches embryonnaires humaines, et soient sur un support, dans un milieu de culture approprié. Ladite différenciation mène à : - soit un premier groupe de cellules différenciées spécialisées dans des conditions normoxiques, et dans un milieu de culture approprié, - soit un second groupe de cellules différenciées spécialisées dans des conditions hypoxiques, dans un milieu de culture de même nature que celui utilisé pour obtenir le premier groupe de cellules différenciées spécialisées, lesdits premier et second groupes de cellules différenciées spécialisées retenant les propriétés fonctionnelles des cellules différenciées spécialisées correspondantes obtenues respectivement par un procédé biologique naturel.

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