Cellular receptor for hiv-1 vpr essential for g2/m phase...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) A61K 48/00 (2006.01) A61P 35/00 (2006.01) C07H 21/04 (2006.01) C07K 14/47 (2006.01) C07K 14/705 (2006.01) C07K 16/18 (2006.01) C12N 15/00 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2306440

The present invention is directed to substantially pure Human Vpr Interacting Protein (hVIP), and fragments thereof. Also disclosed are isolated nucleic acid molecules that encode hVIP, or a fragment thereof; nucleic acid probes and primers directed to nucleic acid molecules that encode hVIP, or a fragment thereof; oligonucleotide molecules that consist of a nucleotide sequence complementary to a portion of the nucleotide sequence that encodes hVIP; vectors comprising nucleic acid molecules encoding hVIP; recombinant expression vectors that comprise nucleic acid sequences that encode hVIP; host cells that comprise recombinant expression vectors which include nucleic acid sequences that encode hVIP; genetic therapy vectors comprising nucleic acid molecules encoding hVIP; isolated antibody which binds to an epitope on hVIP; pharmaceutical compositions comprising a pharmaceutically acceptable carrier and nucleic acid molecules complementary to a portion of hVIP; methods of making hVIP; and methods of inhibiting expression of hVIP oligonucleotides complementary to a portion of the nucleotide sequence that encodes hVIP.

La présente invention concerne une proteine Vpr humaine d'interaction (hVIP) sensiblement pure et ses fragments. On décrit des molécules isolées d'acide nucléique codant hVIP ou un fragment desdites molécules; des sondes et des amorces d'acide nucléique dirigées vers des molécules d'acide nucléique codant hVIP ou un fragment desdites sondes et amorces; des molécules oligonucléotides constituées d'une séquence nucléotidique complémentaire d'une partie de la séquence nucléotidique codant hVIP. On décrit également des vecteurs comprenant des molécules d'acide nucléique codant hVIP; des vecteurs d'expression recombinants comprenant des séquences d'acide nucléique codant hVIP; des cellules hôtes comprenant des vecteurs d'expression recombinants eux-mêmes contenant des séquences d'acide nucléique codant hVIP; des vecteurs de thérapie génique comprenant des molécules d'acide nucléique codant hVIP; un anticorps isolé qui se fixe à un épitope de hVIP. On décrit enfin des compositions pharmaceutiques comprenant un porteur pharmaceutiquement acceptable et des molécules d'acide nucléique complémentaires d'une partie de hVIP; des procédés de production de hVIP; et des procédés permettant d'inhiber l'expression des oligonucléotides hVIP complémentaires d'une partie de la séquence nucléotidique codant hVIP.

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